March 2008
Leap day
I think we made pretty good use of the extra day this year. Found some Laby’s in sea fans. Cranked out some abalone real-time (starting with mere tissue this morning) Characterized cyp 450 in developing oysters & now know their REAL RNA concentrations. Ate plenty of bagels. Got some presumptive OMPs off to ASU. And Cullen had a real nice ladder on his gel today. SR
Awards
PhD student Eric Larson (supervisor Julian Olden) was recently awarded an EAPSI (East Asia and Pacific Summer Institutes) Fellowship from the National Science Foundation. The award provides a stipend and covers travel/lodging costs for Eric to conduct invasive species research in Japan for 2 months this summer. MS student Jessica Silver (supervisor Claire Horner-Devine) recently was awarded 3...
February 2008
16th Conference for Shellfish Growers – Shellfish... →
proposal (draft) finished! + sequences and pcr
A draft of the proposal is done! It took a while to find good references, but it was worth it. I looked at sequences that were sent off a couple weeks back. The four genomic sequences of TOLLIP were garbage… three didn’t have any results and one had the last 50 bp of the sequence I already know. But the four cDNA samples with the new primer had some good results. I got about an...
FedEx: Sea fans are on the way. →
Watch
Watch
Fine structure of the early stages of... →
“I routinely used UV to visualize restriction digest bands that I then extracted...”
– Turn Away from the (UV) Light | Bitesize Bio
Arctic Adventure! Ocean Tales of Currents and... →
“BMP-1/TLD proteases plays a critical role in the activation of latent myostatin...”
– Genetic analysis of the role of proteolysis in the…[PLoS ONE. 2008] - PubMed Result
inbox zero
Yes, the sequences are not the brightest point of the week, though I have something that at first seemed just as disasterous. I deleted about 3000 of my most recent emails. However after thinking about it I always wanted to get by inbox to zero but was afraid. This was my excuse and I just wiped everything cleaned and will follow the concepts described below (click on the photo). If you have the...
Bad dreams...
The quarter must be winding up, I’m dreaming about this place. My sequences are back. No what I was hoping for… Prof Roberts and Sam are going to try cloning the bands from the OmpA gels in hopes of getting more product for better sequences. Sam got sequences back from water with no primer added. Yeah, weird. Maybe we have special DNA here in ROberts lab that acts like yeast, and...
Rapid transcriptome characterization for a... →
Faculty Scholarly Productivity Index →
UW Goes Green →
Professors and students are excitedly working away on environmental issues in small pockets across the University of Washington. Now the school is looking to connect those labs, offices and classrooms under one common green umbrella. KPLU’s Chana Joffe-Walt explains
I wish computer screens looked like Jessica Alba
If so, then I wouldn’t mind staring at them all day! But honestly, I’ve been doing online research, reading .pdf articles, and typing up my proposal for the past two weeks. It’s getting to the point where a trip to the library to get a BOOK is exciting! I don’t mean to complain because I’m definitely learning more about withering syndrome in abalone, the history of...
“Claire Horner-Devine launched a census of marine microbes in Hood Canal”
– uweek.org | Cruise information plays part in Sound solutions
FISH 310 →
Spring is on its way!
“The 2008 Thermostable Polymerase Awards (the THEPA’s) are underway and you have...”
– The Best Polymerases of 2008 | Bitesize Bio
WatchDiscovering Biology in a Digital World
“vitellogenin synthesis in the Pacific abalone”
– Ovarian follicle cells are the site of vitellogeni…[Comp Biochem Physiol A Mol Integr Physiol. 2008] - PubMed Result
“The complete cDNA sequence of macrophage expressed gene (saMpeg1), a...”
– Molecular cloning and responsive expression of mac…[Fish Shellfish Immunol. 2007] - PubMed Result
WatchI vote we paint the walls with this (will save money on white boards) —SR
Stop sharing spreadsheets, start collecting... →
UW Climate Survey →
go ahead and take it..
“A potential performance enhancement strategy?”
– [Scand J Med Sci Sports. 2008] - PubMed Result
Gels....
My advice on gels, be sure you add enough ethidium bromide… Last weeks gels were good and bad. I got a band off the OMPA and it looked to be the right size!! Going off to be sequenced today. I’m running a PCR today using new primers for the 16s gene of RLP. Seeing as this product will be over 500bp long, it will be much easier to see than the previous primers that produced a product...
“when male brains are presented with a video game, they show greater activation...”
– Video games, the male brain, and addiction
So as Dr. Roberts mentioned, abalone TOLLIP with introns is being sent out for sequencing today. My PCR reactions from last week produced two nice products. I had other gDNA reactions with forward degenerate primers and a new forward primer farther upstream (866F), but no bands. That’s probably because the products were too long for the genomic pol (or the extension phase wasn’t long...
The Steven Berkeley Marine Conservation Fellowship
This fellowship was created by AFS in 2007 to honor the memory of Steven Berkeley, a dedicated fisheries scientist with a passionate interest in integrating the fields of marine ecology, conservation biology, and fisheries science to improve fisheries management. Berkeley was a long-time member of AFS and a member of the first Board of Directors of the Fisheries Conservation Foundation. The...
a few tweaks on the white pages.. →
SAFS Newsletter - Focus on Molecular Faculty →
“Precision instruments they may be, but the accuracy of micro-pipettes depends on...”
– 17 Ways to Stop Pipetting Errors Ruining Your Experiments | Bitesize Bio 