gene|fish

If perfect doubling occurs with each amplification cycle, the spacing of the fluorescence curves will be determined by the equation 2n = dilution factor, where n is the number of cycles between curves (in other words, the difference between the curves’ CT values). For example, with a 10-fold serial dilution of DNA, n^2 = 10. Therefore, n = 3.32 and the CT values should be separated by approximately 3.32 cycles.